Click on a slide to go to that topic.
Calmodulin regulation of Ca2+ channels viewed by FRET-based microscopy 00:00:00 A most important contribution of the physicist? a unique way of seeing the biological landscape 00:01:03 Unique way of seeing?case examples in calmodulin regulation of Ca2+ channels 00:01:25 Ca2+ signals?a lingua franca of life at the molecular and cellular level 00:02:08 Ca2+ channels: important Ca2+ signal transducers 00:03:13 00:04:18 00:06:19 00:08:22 00:09:40 00:10:02 Quantifying Ca2+-dependent facilitation of P/Q-type (a1A) channels (1) 00:11:28 00:12:34 00:13:01 00:14:11 00:15:40 00:17:28 00:18:32 P/Q-type (a1A) channel facilitation and inactivation: CaM lobe-specific bifurcation of local Ca2+ and putative preassociation 00:18:55 00:20:28 CaM bifurcation of local Ca2+ signal involves lobe-specific decoding? 00:21:29 Unique way of seeing?case examples in calmodulin regulation of Ca2+ channels 00:24:05 CaM bifurcation of local Ca2+ signal involves lobe-specific decoding? 00:24:30 Unique way of seeing?case examples in calmodulin regulation of Ca2+ channels 00:26:17 Much of molecular-level communication involves orchestrated protein-protein interaction 00:28:01 FRET assays of protein-protein interaction in living cells 00:28:32 Aequorea victoria and GFP 00:28:50 Principles of Fluorescence Spectroscopy 00:30:26 Fluorescence Resonance Energy Transfer (FRET) 00:31:05 Principles of FRET: Basis of a molecular yardstick 00:31:51 Challenges for detecting static FRET 00:32:31 Novel 33-FRET Assay: System analysis 00:33:31 Novel 33-FRET Assay: Simple description 00:34:04 Sensitive and selective detection with 33-FRET 00:35:23 ?1C YFP targets the surface membrane 00:36:09 apoCaM preassociates with L-type channels in situ 00:36:38 How well does apoCaM preassociate with L-type channels? 00:37:32 apoCaM preassociates with L-type channels in situ 00:37:37 How well does apoCaM preassociate with L-type channels? 00:37:56 Inferring binding properties in situ 00:38:35 CaM may preassociate at a similar location across HVA family 00:39:28 Where does CaM preassociate? 00:40:18 Where does CaM preassociate? FRET-based two-hybrid mapping in the ultimate biological cuvette 00:40:45 Distinguishing relative affinity helps identify the apoCaM pocket 00:41:09 Where does CaM preassociate? FRET-based two-hybrid mapping in the ultimate biological cuvette 00:41:41 Distinguishing relative affinity helps identify the apoCaM pocket 00:41:46 The IQ domain is crucial to the binding pocket 00:42:02 00:42:28 Acknowledgements 00:42:48
