Slide 002: "A most important contribution of the physicist? a unique way of seeing the biological landscape" [ 00:01:03 ]

Go To Slide: Calmodulin regulation of Ca2+ channels viewed by FRET-based microscopy A most important contribution of the physicist? a unique way of seeing the biological landscape Unique way of seeing?case examples in calmodulin regulation of Ca2+ channels Ca2+ signals?a lingua franca of life at the molecular and cellular level Ca2+ channels: important Ca2+ signal transducers Quantifying Ca2+-dependent facilitation of P/Q-type (a1A) channels (1) P/Q-type (a1A) channel facilitation and inactivation: CaM lobe-specific bifurcation of local Ca2+ and putative preassociation CaM bifurcation of local Ca2+ signal involves lobe-specific decoding? Unique way of seeing?case examples in calmodulin regulation of Ca2+ channels CaM bifurcation of local Ca2+ signal involves lobe-specific decoding? Unique way of seeing?case examples in calmodulin regulation of Ca2+ channels Much of molecular-level communication involves orchestrated protein-protein interaction FRET assays of protein-protein interaction in living cells Aequorea victoria and GFP Principles of Fluorescence Spectroscopy Fluorescence Resonance Energy Transfer (FRET) Principles of FRET: Basis of a molecular yardstick Challenges for detecting static FRET Novel 33-FRET Assay: System analysis Novel 33-FRET Assay: Simple description Sensitive and selective detection with 33-FRET ?1C YFP targets the surface membrane apoCaM preassociates with L-type channels in situ How well does apoCaM preassociate with L-type channels? apoCaM preassociates with L-type channels in situ How well does apoCaM preassociate with L-type channels? Inferring binding properties in situ CaM may preassociate at a similar location across HVA family Where does CaM preassociate? Where does CaM preassociate? FRET-based two-hybrid mapping in the ultimate biological cuvette Distinguishing relative affinity helps identify the apoCaM pocket Where does CaM preassociate? FRET-based two-hybrid mapping in the ultimate biological cuvette Distinguishing relative affinity helps identify the apoCaM pocket The IQ domain is crucial to the binding pocket Acknowledgements